effectene transfection reagent (Qiagen)
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Qiagen
effectene transfection reagent
Effectene Transfection Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/effectene+transfection+reagent/effectene+transfection+reagent/pm40660165-205-15-18
Average 90 stars, based on 1 article reviews
Effectene Transfection Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/effectene+transfection+reagent/effectene+transfection+reagent/pm40660165-205-15-18
Average 90 stars, based on 1 article reviews
effectene transfection reagent - by Bioz Stars,
2026-10
90/100 stars
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Plasmid Preparation:Article Title: Cellular Lyso-Gb3 Is a Biomarker for Mucolipidosis II. Article Snippet: The double-stranded DNA coding guide RNA sequences were prepared by annealing synthesized complementary DNA oligo pairs on a thermal cycler after phosphorylation by the T4 polynucleotide kinase (TaKaRa Bio, Shiga, Japan) and then cloned at the BbsI site of the px458 plasmid. .. The targeting plasmid was transfected into HeLa Kyoto cells with the Article Title: Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout. Article Snippet: .. Cells were transfected with 0.08 μg of pCMVd1-NLS-Gal4-iLight-VP16 (or empty pcDNA3.1+ plasmid) and 0.32 μg of pAAV2-G12-AkaLuc-P2AProinsulin (0.4 μg per well) using Article Title: Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila. Article Snippet: Drosophila Schneider S2 cells were maintained at 24 °C in CCM3 medium (Hyclone). .. For each (co-) transfection, two million cells were transfected with 4 μg plasmid DNA using Article Title: Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout Article Snippet: .. Cells were transfected with 0.08 μg of pCMVd1-NLS-Gal4-iLight-VP16 (or empty pcDNA3.1+ plasmid) and 0.32 μg of pAAV2-G12-AkaLuc-P2A-Proinsulin (0.4 μg per well) using Transfection:Article Title: Cellular Lyso-Gb3 Is a Biomarker for Mucolipidosis II. Article Snippet: The double-stranded DNA coding guide RNA sequences were prepared by annealing synthesized complementary DNA oligo pairs on a thermal cycler after phosphorylation by the T4 polynucleotide kinase (TaKaRa Bio, Shiga, Japan) and then cloned at the BbsI site of the px458 plasmid. .. The targeting plasmid was transfected into HeLa Kyoto cells with the Article Title: Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout. Article Snippet: .. Cells were transfected with 0.08 μg of pCMVd1-NLS-Gal4-iLight-VP16 (or empty pcDNA3.1+ plasmid) and 0.32 μg of pAAV2-G12-AkaLuc-P2AProinsulin (0.4 μg per well) using Article Title: Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila. Article Snippet: Drosophila Schneider S2 cells were maintained at 24 °C in CCM3 medium (Hyclone). .. For each (co-) transfection, two million cells were transfected with 4 μg plasmid DNA using Article Title: Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila Article Snippet: .. Briefly, S2 cells at about 2.0 × 10 6 cells/ml were diluted 1:1 in culture medium and cultured for 16–20 h. Cells were transfected with dsRNAs using Article Title: Female-germline specific protein Sakura interacts with Otu and is crucial for germline stem cell renewal and differentiation and oogenesis Article Snippet: Transient protein expression in S2 cells was performed using the pAc5.1/V5-HisB plasmid vector (Invitrogen). .. A total of 1 μg plasmids were transfected using the Article Title: Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila. Article Snippet: .. Briefly, S2 cells at about 2.0 × 106 cells/ml were diluted 1:1 in culture medium and cultured for 16–20 h. Cells were transfected with dsRNAs using Article Title: Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout Article Snippet: .. Cells were transfected with 0.08 μg of pCMVd1-NLS-Gal4-iLight-VP16 (or empty pcDNA3.1+ plasmid) and 0.32 μg of pAAV2-G12-AkaLuc-P2A-Proinsulin (0.4 μg per well) using Article Title: Dissection of DNA damage and repair pathways in live cells by femtosecond laser irradiation and free electron modelling Article Snippet: .. For recruitment experiments, cells were transfected with expression plasmids as indicated using Cotransfection:Article Title: Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila. Article Snippet: Drosophila Schneider S2 cells were maintained at 24 °C in CCM3 medium (Hyclone). .. For each (co-) transfection, two million cells were transfected with 4 μg plasmid DNA using Cell Culture:Article Title: Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila Article Snippet: .. Briefly, S2 cells at about 2.0 × 10 6 cells/ml were diluted 1:1 in culture medium and cultured for 16–20 h. Cells were transfected with dsRNAs using Article Title: Adar contributes to genome integrity by regulating R-loop homeostasis in Drosophila. Article Snippet: .. Briefly, S2 cells at about 2.0 × 106 cells/ml were diluted 1:1 in culture medium and cultured for 16–20 h. Cells were transfected with dsRNAs using Expressing:Article Title: Dissection of DNA damage and repair pathways in live cells by femtosecond laser irradiation and free electron modelling Article Snippet: .. For recruitment experiments, cells were transfected with expression plasmids as indicated using |